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Project Information
Project Title
Structure–Activity Relationship and Molecular Docking of Genistein for Enhanced Anti-Inflammatory Potential
Activity Type
Research Assistant
Program Model
Plan A: GLIP
Eligibility: Exclusively for undergraduate students.
Duration: 1 semester or 3–4 months (depending on the international student's academic calendar) between November 2025 – August 2026.
University Financial Support:
- Tuition fees and other university fees
- On-campus accommodation fees (excluding electricity charges)
- Daily allowance for international students (240 Baht/day)
Eligibility: Exclusively for undergraduate students.
Duration: 1 semester or 3–4 months (depending on the international student's academic calendar) between November 2025 – August 2026.
University Financial Support:
- Tuition fees and other university fees
- On-campus accommodation fees (excluding electricity charges)
- Daily allowance for international students (240 Baht/day)
Duration
February 2026 - August 2026 (7 months)
Student Qualification
1. Currently enrolled in an undergraduate program in Chemistry, Medicinal Chemistry, Pharmaceutical Sciences, or related fields.
2. Completed foundational courses such as Organic Chemistry and Medicinal Chemistry (or equivalent).
3. Able to communicate effectively in English in both academic and laboratory settings.
4. Demonstrated interest in drug design or structure–activity relationship (SAR) research.
5. Possess basic computer skills and willingness to learn computational tools.
6. Applicants with prior knowledge or experience in in silico techniques (e.g., molecular docking, molecular visualization, ADME prediction) will be given special consideration.
2. Completed foundational courses such as Organic Chemistry and Medicinal Chemistry (or equivalent).
3. Able to communicate effectively in English in both academic and laboratory settings.
4. Demonstrated interest in drug design or structure–activity relationship (SAR) research.
5. Possess basic computer skills and willingness to learn computational tools.
6. Applicants with prior knowledge or experience in in silico techniques (e.g., molecular docking, molecular visualization, ADME prediction) will be given special consideration.
Project Status
Approved
Activity Details
Overview
This project focuses on enhancing the anti-inflammatory potential of Genistein through an integrated study of its structure–activity relationship and molecular interactions with key inflammatory targets. The research involves designing a series of Genistein derivatives and evaluating their predicted binding affinity to LOX and NF-κB–related proteins using molecular docking. Promising candidates from the computational analysis will then be synthesized and subjected to biological evaluation, including LOX inhibition assays and assessment of inflammatory gene expression in RAW 264.7 cells. The findings will generate valuable SAR insights and identify high-potential Genistein derivatives that could serve as lead compounds for the development of future anti-inflammatory therapeutics or health products.
Weekly Plan
| Week | Activity |
|---|---|
| 1 | Literature Review & Project Preparation - Review current knowledge on Genistein, SAR, anti-inflammatory mechanisms, LOX and NF-κB pathways. |
| 2 | Literature Review & Project Preparation - Review current knowledge on Genistein, SAR, anti-inflammatory mechanisms, LOX and NF-κB pathways. |
| 3 | Synthesis of Genistein Derivatives - Develop synthetic pathways and procure required chemicals |
| 4 | Synthesis of Genistein Derivatives - Continue synthesis; perform reaction optimization as needed - Purify products using chromatography and verify structural integrity - Characterize synthesized compounds (NMR, MS, IR). |
| 5 | Synthesis of Genistein Derivatives - Continue synthesis; perform reaction optimization as needed - Purify products using chromatography and verify structural integrity - Characterize synthesized compounds (NMR, MS, IR). |
| 6 | Synthesis of Genistein Derivatives - Continue synthesis; perform reaction optimization as needed - Purify products using chromatography and verify structural integrity - Characterize synthesized compounds (NMR, MS, IR). |
| 7 | Synthesis of Genistein Derivatives - Continue synthesis; perform reaction optimization as needed - Purify products using chromatography and verify structural integrity - Characterize synthesized compounds (NMR, MS, IR). |
| 8 | Synthesis of Genistein Derivatives - Continue synthesis; perform reaction optimization as needed - Purify products using chromatography and verify structural integrity - Characterize synthesized compounds (NMR, MS, IR). |
| 9 | Biological Evaluation - Conduct LOX inhibition assays using synthesized derivatives |
| 10 | Biological Evaluation - Conduct LOX inhibition assays using synthesized derivatives - Culture RAW 264.7 macrophage cells; perform cytotoxicity screening |
| 11 | Biological Evaluation - Culture RAW 264.7 macrophage cells; perform cytotoxicity screening |
| 12 | Biological Evaluation - Culture RAW 264.7 macrophage cells; perform cytotoxicity screening |
| 13 | Biological Evaluation - Measure expression of inflammatory-related genes (e.g., COX-2, TNF-α, IL-6) after compound treatment. - Analyze qPCR or RT-PCR results to validate anti-inflammatory effects. |
| 14 | Biological Evaluation - Measure expression of inflammatory-related genes (e.g., COX-2, TNF-α, IL-6) after compound treatment. - Analyze qPCR or RT-PCR results to validate anti-inflammatory effects. |
| 15 | Data Integration, SAR Analysis & Reporting - Identify structural features linked to improved anti-inflammatory activity - Prepare Final report |
| 16 | Data Integration, SAR Analysis & Reporting - Prepare Final report - Prepare presentation slides and submit final report |
Project Manager
Full Name (EN)
Jira Jongcharoenkamol
Faculty / Institute
Faculty of Pharmaceutical Sciences
Email
jiraj@nu.ac.th